Multiparty Quantum Secret Sharing

Based on a quantum secure direct communication (QSDC) protocol [Phys. Rev. A69(04)052319], we propose a $(n,n)$-threshold scheme of multiparty quantum secret sharing of classical messages (QSSCM) using only single photons. We take advantage of this multiparty QSSCM scheme to establish a scheme of multiparty secret sharing of quantum information (SSQI), in which only all quantum information receivers collaborate can the original qubit be reconstructed. A general idea is also proposed for constructing multiparty SSQI schemes from any QSSCM scheme

Phased mission reliability analysis of unmanned ship systems

With the development of unmanned ships, their use in production is becoming more and more common. However, the unmanned ship work cycle is long and the work environment is complex, and it is still very difficult to calculate the phased mission reliability without unmanned ship. We analyze the unmanned ship phased mission reliability based on the binary decision diagram. Moreover, redundancy is used as the unmanned ship reliability optimization scheme. Considering the resource limitation, and the capacity of unmanned ship, the redundancy allocation scheme of unmanned ship is established. The redundancy allocation scheme is solved by marginal optimization algorithm. Finally, a case study is established to analyze the effectiveness and practicality of the proposed method

7-Isopropyl-1,4a-dimethyl-1,2,3,4,4a,5,6,7,8,9,10,10a-dodeca­hydro­phenan­threne-1-carboxylic acid

The title compound, C20H32O2, has been isolated from hydrogenated rosin. There are two independent mol­ecules in the asymmetric unit. In each mol­ecule, the cyclo­hexane ring assumes a chair conformation, while the two cyclo­hexene rings adopt half-chair and envelope conformations. Inter­molecular O—H⋯O hydrogen bonding between carboxyl groups links pairs of independent mol­ecules into dimers

Identification of New Glutamate Decarboxylases from \u3cem\u3eStreptomyces\u3c/em\u3e for Efficient Production of γ-Aminobutyric Acid in Engineered \u3cem\u3eEscherichia coli\u3c/em\u3e

Background Gamma (γ)-Aminobutyric acid (GABA) as a bioactive compound is used extensively in functional foods, pharmaceuticals and agro-industry. It can be biosynthesized via decarboxylation of monosodium glutamate (MSG) or L-glutamic acid (L-Glu) by glutamate decarboxylase (GAD; EC4.1.1.15). GADs have been identified from a variety of microbial sources, such as Escherichia coli and lactic acid bacteria. However, no GADs from Streptomyces have been characterized. The present study is aimed to identify new GADs from Streptomyces strains and establish an efficient bioproduction platform for GABA in E. coli using these enzymes. Results By sequencing and analyzing the genomes of three Streptomycesstrains, three putative GADs were discovered, including StGAD from Streptomyces toxytricini NRRL 15443, SsGAD from Streptomyces sp.MJ654-NF4 and ScGAD from Streptomyces chromofuscus ATCC 49982. The corresponding genes were cloned from these strains and heterologously expressed in E. coli BL21(DE3). The purified GAD proteins showed a similar molecular mass to GadB from E. coliBL21(DE3). The optimal reaction temperature is 37 °C for all three enzymes, while the optimum pH values for StGAD, SsGAD and ScGAD are 5.2, 3.8 and 4.2, respectively. The kinetic parameters including Vmax, Km, kcat and kcat/Km values were investigated and calculated through in vitro reactions. SsGAD and ScGAD showed high biocatalytic efficiency with kcat/Km values of 0.62 and 1.21 mM− 1·s− 1, respectively. In addition, engineered E. coli strains harboring StGAD, SsGAD and ScGAD were used as whole-cell biocatalysts for production of GABA from L-Glu. E. coli/SsGAD showed the highest capability of GABA production. The cells were repeatedly used for 10 times, with an accumulated yield of 2.771 kg/L and an average molar conversion rate of 67% within 20 h. Conclusions Three new GADs have been functionally characterized from Streptomyces, among which two showed higher catalytic efficiency than previously reported GADs. Engineered E. coli harboring SsGAD provides a promising cost-effective bioconversion system for industrial production of GABA

Research on the Effect of Non-Thermal Plasma on the Sterilization of Craft Beer

The craft beer was continuously sterilized by the non-thermal plasma reactor, and its sterilization and quality were evaluated. The total count of aerobic plate, yeast and Escherichia coli were used as microbial indexes, the effects of plasma sterilization and pasteurization on the bactericidal effect of craft beer were studied. Meanwhile, the beer color, foam hold and pH were evaluated to obtain the optimum sterilization condition with the response surface. The results showed that, compared with pasteurization, the sterilization rate after plasma treatment was almost the same, but the chroma, brightness and bubble retention were better, which was closer to the original beer. The total number of colonies and Escherichia coli were almost completely killed, and the number of yeast was reduced by 97.5% with the 40 kV plasma treatment voltage, 10 s and 60 mL/min gas flow rate. According to the analysis of Design Expert software, the sterilization effect and brightness were the best when the voltage, plasma treatment times and gas flow were 40 kV, 10 s and 60 mL/min, respectively. The predicted values were consistent with the measured values. The influence of each factors were voltage > time > gas flow rate

N-Benzyl­idenenordehydro­abietylamine

The title compound [systematic name: (1R,4aS,10aR,E)-N-benzyl­idene-7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octa­hydro­phenanthren-1-amine], C26H33N, has been synthesized from nor-dehydro­abietylamine and benzaldehyde. The two cyclo­hexane rings form a trans ring junction with classic chair and half-chair conformations, respectively, the two methyl groups are on the same side of tricyclic hydro­phenanthrene structure. The dihedral angle between two benzene rings is 44.2 (4)°. The C=N bond is in an E configuration

Exogenous Application of a Plant Elicitor Induces Volatile Emission in Wheat and Enhances the Attraction of an Aphid Parasitoid Aphidius gifuensis.

peer reviewedIt is well known that plant elicitors can induce plant defense against pests. The herbivore-induced plant volatile (HIPV) methyl salicylate (MeSA), as a signaling hormone involved in plant pathogen defense, is used to recruit natural enemies to protect wheat and other crops. However, the defense mechanism remains largely unknown. Here, the headspace volatiles of wheat plants were collected and analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography with electroantennographic detection (GC-EAD) and principal component analysis (PCA). The results showed that exogenous application of MeSA induced qualitative and quantitative changes in the volatiles emitted from wheat plants, and these changes were mainly related to Carveol, Linalool, m-Diethyl-benzene, p-Cymene, Nonanal, D-limonene and 6-methyl-5-Hepten-2-one. Then, the electroantennogram (EAG) and Y-tube bioassay were performed to test the physiological and behavioral responses of Aphidius gifuensis Ashmesd to the active volatile compounds (p-Cymene, m-Diethyl-benzene, Carveol) that identified by using GC-EAD. The female A. gifuensis showed strong physiological responses to 1 μg/μL p-Cymene and 1 μg/μL m-Diethyl-benzene. Moreover, a mixture blend was more attractive to female A. gifuensis than a single compound. These findings suggested that MeSA could induce wheat plant indirect defense against wheat aphids through attracting parasitoid in the wheat agro-ecosystem

Prognostic Value of Vascular Endothelial Growth Factor Expression in Patients with Lung Cancer: A Systematic Review with Meta-Analysis

BackgroundVascular endothelial growth factor (VEGF) has been implicated in tumorigenesis and metastasis, and it presumably mediates the proliferation of endothelial cells and promotes vascular permeability. However, the prognostic value of VEGF overexpression in patients with lung cancer remains controversial.MethodsSurvival data from published studies were aggregated following a methodological assessment. A systematic review of eligible studies with meta-analysis was performed to quantitatively review the correlation of VEGF overexpression with survival in patients with lung cancer.ResultsWe conducted a final analysis of 5386 patients from 51 studies. The studies were categorized by histology, disease stage, patient race, VEGF isoform, and laboratory techniques used. Combined hazard ratios suggested that VEGF overexpression had an unfavorable impact on survival of patients with non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). However, VEGFC and vascular endothelial growth factor receptor 3 (VEGFR3)/flt-1 overexpression did not significantly correlate with survival in patients with NSCLC. In stage I–III NSCLC with VEGF, the hazard ratio (95% confidence interval) was 1.46 (1.38–1.54) overall, 1.35 (1.24–1.46) in Asian patients, 1.61 (1.49–1.73) in non-Asian patients, 1.41 (1.17–1.65) in SCLC, 1.27 (1.06–1.47) in adenocarcinoma, 1.57 (1.43–1.70) in stage I NSCLC, 1.46 (1.38–1.55) in NSCLC by immunohistochemistry, 1.52 (1.23–1.81) in NSCLC by reverse transcription-polymerase chain reaction, 1.22 (0.96–1.47) in NSCLC with VEGFC, and 1.58 (0.96–2.20) in NSCLC with VEGFR3/flt-1. The data collected were not sufficient to determine the prognostic value of VEGF in patients with squamous cell lung carcinomas.ConclusionVEGF overexpression indicates a poor prognosis for patients with NSCLC and SCLC; VEGFC and VEGFR3/flt-1 overexpression was not significantly correlated with survival for patients with NSCLC